This church is located on a hill above Karies Village, about 6km from Chios Town. It was founded in 1886 by monk Parthenios who also is buried inside the monastery. The church of Agios Markos in Chios played an important role during the struggle for freedom till 1912, when the island was finally joined to Greece. Also in the early 20th century, a school of hagiography was working there. The monastery offers great views of Chios Town and the surrounding areas.